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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmEGFP_N_dest.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCHMP6
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SpeciesH. sapiens (human)
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Entrez GeneCHMP6 (a.k.a. VPS20)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CHMP6_GFP was a gift from Daniel Gerlich (Addgene plasmid # 31806 ; http://n2t.net/addgene:31806 ; RRID:Addgene_31806) -
For your References section:
Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Guizetti J, Schermelleh L, Mantler J, Maar S, Poser I, Leonhardt H, Muller-Reichert T, Gerlich DW. Science. 2011 Mar 25;331(6024):1616-20. Epub 2011 Feb 10. 10.1126/science.1201847 PubMed 21310966