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pCDF1-GRM3 (E870K)
(Plasmid #31802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF1- MCS2-EF1-puromycin
  • Backbone manufacturer
    SBI
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GRM3
  • Alt name
    mGluR3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2640
  • Mutation
    Changed glutamic acid 870 to lysine
  • GenBank ID
    NM_000840
  • Entrez Gene
    GRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF1-GRM3 (E870K) was a gift from Yardena Samuels (Addgene plasmid # 31802 ; http://n2t.net/addgene:31802 ; RRID:Addgene_31802)

  • For your References section:

    Exon capture analysis of G protein-coupled receptors identifies activating mutations in GRM3 in melanoma. Prickett TD, Wei X, Cardenas-Navia I, Teer JK, Lin JC, Walia V, Gartner J, Jiang J, Cherukuri PF, Molinolo A, Davies MA, Gershenwald JE, Stemke-Hale K, Rosenberg SA, Margulies EH, Samuels Y. Nat Genet. 2011 Sep 25;43(11):1119-26. doi: 10.1038/ng.950. 10.1038/ng.950 PubMed 21946352
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