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Purpose3rd gen lentiviral vector for expression of Reverse Tetracycline transactivator 3G (rtTA3G).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE
- Backbone size w/o insert (bp) 8054
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namertTA3G
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Alt nameReverse Tetracycline transactivator 3G
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Insert Size (bp)746
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVforw
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byThis plasmid was made by Dr. Dominic Esposito (SAIC-Frederick)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV rtTA3G Blast (R980-M38-658) was a gift from Dominic Esposito (Addgene plasmid # 31797)