-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 31796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepegfp-N1
-
Backbone manufacturerClontech
- Backbone size (bp) 4722
-
Modifications to backboneGateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
-
Vector typeMammalian Expression
- Promoter CMV
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDest-eGFP-N1 was a gift from Robin Shaw (Addgene plasmid # 31796 ; http://n2t.net/addgene:31796 ; RRID:Addgene_31796) -
For your References section:
BIN1 localizes the L-type calcium channel to cardiac T-tubules. Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, Colecraft HM, Shaw RM. PLoS Biol. 2010 Feb . 8(2):e1000312. 10.1371/journal.pbio.1000312 PubMed 20169111