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pcDNA3.1/HisC-mTAZ
(Plasmid #31793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/His C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAZ
  • Alt name
    WWTR1
  • Species
    M. musculus (mouse)
  • GenBank ID
    CAC17733.1
  • Entrez Gene
    Wwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
  • Promoter CMV
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Xpress Epitope Tag (N terminal on insert)
    • EK (Enterokinase) Recognition Site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/HisC-mTAZ was a gift from Michael Yaffe (Addgene plasmid # 31793 ; http://n2t.net/addgene:31793 ; RRID:Addgene_31793)

  • For your References section:

    TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Hong JH, Hwang ES, McManus MT, Amsterdam A, Tian Y, Kalmukova R, Mueller E, Benjamin T, Spiegelman BM, Sharp PA, Hopkins N, Yaffe MB. Science. 2005 Aug 12;309(5737):1074-8. 10.1126/science.1110955 PubMed 16099986
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