pT3-myr-AKT-HA
(Plasmid #31789)

• Depositing Lab: Xin Chen
• Publication: Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-83. Epub 2010 Dec 11.

Backbone

• Vector backbone: pT3-EF1a
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Akt1
• Alt name: Akt
• Species: M. musculus (mouse)
• Insert Size (bp): 1503
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Tags / Fusion Proteins:
  • Myr (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF-1a-Fwd
• 3′ sequencing primer: pT3EF1a-R 5'-TGACACCTACTCAGACAATG

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.