pENTR-N90-beta-catenin
(Plasmid #31787)

• Depositing Lab: Xin Chen
• Publication: Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4.

Backbone

• Vector backbone: pENTR1A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2700
• Vector type: Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-catenin
• Alt name: b-catenin
• Alt name: CTNNB1
• Species: H. sapiens (human)
• Insert Size (bp): 2121
• Mutation: Deletion of aa 1-90- Constitutively Active
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: pENTR-Fwd
• 3′ sequencing primer: pENTR-Rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry