pcDNA4-TO-myc-His Trask [YdeltaF] mutant
(Plasmid
#31769)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4-TO-myc-His
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5151
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrask
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SpeciesH. sapiens (human)
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MutationY707/734/743F
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Entrez GeneCDCP1 (a.k.a. CD318, SIMA135, TRASK)
- Promoter CMV
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Tags
/ Fusion Proteins
- Myc (C terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4-TO-myc-His Trask [YdeltaF] mutant was a gift from Mark Moasser (Addgene plasmid # 31769 ; http://n2t.net/addgene:31769 ; RRID:Addgene_31769) -
For your References section:
The structural features of Trask that mediate its anti-adhesive functions. Spassov DS, Ahuja D, Wong CH, Moasser MM. PLoS One. 2011 Apr 29;6(4):e19154. 10.1371/journal.pone.0019154 PubMed 21559459