pcDNA4-TO-myc-His wt-Trask
(Plasmid #31768)

• Depositing Lab: Mark Moasser
• Publication: Spassov et al PLoS One. 2011 Apr 29;6(4):e19154.

Backbone

• Vector backbone: pcDNA4-TO-myc-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5151
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trask
• Species: H. sapiens (human)
• Entrez Gene: CDCP1 (a.k.a. CD318, SIMA135, TRASK)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: BGH-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry