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pcDNA4-TO-myc-His wt-Trask
(Plasmid #31768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4-TO-myc-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5151
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trask
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDCP1 (a.k.a. CD318, SIMA135, TRASK)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-TO-myc-His wt-Trask was a gift from Mark Moasser (Addgene plasmid # 31768 ; http://n2t.net/addgene:31768 ; RRID:Addgene_31768)

  • For your References section:

    The structural features of Trask that mediate its anti-adhesive functions. Spassov DS, Ahuja D, Wong CH, Moasser MM. PLoS One. 2011 Apr 29;6(4):e19154. 10.1371/journal.pone.0019154 PubMed 21559459
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