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pDONR221-MICU1-sh1-rORF
(Plasmid #31764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 1874
  • Vector type
    pDONR Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MICU1
  • Alt name
    CBARA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • Mutation
    RNAi-resistant ORF (rORF). 8 synonymous changes in b.p. 1272-1293 downstream from ATG for RNAi resistance.
  • GenBank ID
    NM_001195518
  • Entrez Gene
    MICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
  • Promoter None
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 (-21) Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-MICU1-sh1-rORF was a gift from Vamsi Mootha (Addgene plasmid # 31764 ; http://n2t.net/addgene:31764 ; RRID:Addgene_31764)

  • For your References section:

    MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptake. Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK. Nature. 2010 Sep 16;467(7313):291-6. Epub 2010 Aug 8. 10.1038/nature09358 PubMed 20693986
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