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Addgene

pBMN FKBP(DD)-YFP
(Plasmid #31763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN
  • Backbone manufacturer
    Garry Nolan
  • Backbone size w/o insert (bp) 6943
  • Vector type
    Retroviral
  • Selectable markers
    HcRed fluorescence behind IRES

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FKBP(DD)-YFP-HA
  • Alt name
    YFP and FKBP12 (destable domain)
  • Alt name
    YFP fused with FK506 binding protein 1A( destable domain)
  • Species
    H. sapiens (human)
  • Mutation
    F36V, L106P on FKBP12
  • GenBank ID
    NM_000801
  • Entrez Gene
    FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
  • Promoter LTR
  • Tags / Fusion Proteins
    • YFP (C terminal on insert)
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBMN specific
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN FKBP(DD)-YFP was a gift from Thomas Wandless (Addgene plasmid # 31763 ; http://n2t.net/addgene:31763 ; RRID:Addgene_31763)

  • For your References section:

    A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. Cell. 2006 Sep 8;126(5):995-1004. 10.1016/j.cell.2006.07.025 PubMed 16959577
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