pMT-GFP-SAK
(Plasmid
#31755)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMT-GFP
- Backbone size w/o insert (bp) 4200
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSak
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SpeciesD. melanogaster (fly)
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MutationA115S
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Entrez GeneSAK (a.k.a. Dmel_CG7186, CG7186, DSak, DmPLK4, DmPlk4, DmSAK, Dmel\CG7186, PLK4, Plk4, SAK/PLK, SAK/PLK4, SAK/Plk4, Sak, Sak/PLK4, Sak/Plk4, plk4, sak)
- Promoter Metallothionein
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT-GFP-SAK was a gift from Ron Vale (Addgene plasmid # 31755 ; http://n2t.net/addgene:31755 ; RRID:Addgene_31755) -
For your References section:
Patronin regulates the microtubule network by protecting microtubule minus ends. Goodwin SS, Vale RD. Cell. 2010 Oct 15;143(2):263-74. 10.1016/j.cell.2010.09.022 PubMed 20946984