Khc73(1-623)-GFP
(Plasmid
#31750)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMTWG
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKhc73
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SpeciesD. melanogaster (fly)
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MutationContains only aa 1-623, L466P
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Entrez GeneKhc-73 (a.k.a. Dmel_CG8183, CG8183, DmKIN73, DmKin73, DmKlp73, Dmel\CG8183, KHC-73, KIF 13A, KIF13A/B, KLP73, Khc 73, Khc73, Kin73, Kinesin-73, anon-EST:fe3C9, khc-73, khc73)
- Promoter Metallothionein
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MT1-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The construct contains an L466P mutation in Khc73 relative to GenBank reference sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Khc73(1-623)-GFP was a gift from Ron Vale (Addgene plasmid # 31750 ; http://n2t.net/addgene:31750 ; RRID:Addgene_31750)