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Khc73(1-547)-GFP
(Plasmid #31749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTWG
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Khc73
  • Species
    D. melanogaster (fly)
  • Mutation
    Contains only aa 1-547
  • Entrez Gene
    Khc-73 (a.k.a. Dmel_CG8183, CG8183, DmKIN73, DmKin73, DmKlp73, Dmel\CG8183, KHC-73, KIF 13A, KIF13A/B, KLP73, Khc 73, Khc73, Kin73, Kinesin-73, anon-EST:fe3C9, khc-73, khc73)
  • Promoter Metallothionein
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The construct contains V308M and H383R mutations in Khc73 relative to GenBank reference sequences and is as used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Khc73(1-547)-GFP was a gift from Ron Vale (Addgene plasmid # 31749 ; http://n2t.net/addgene:31749 ; RRID:Addgene_31749)

  • For your References section:

    Kinesin-73 is a processive motor that localizes to Rab5-containing organelles. Huckaba TM, Gennerich A, Wilhelm JE, Chishti AH, Vale RD. J Biol Chem. 2011 Mar 4;286(9):7457-67. Epub 2010 Dec 18. 10.1074/jbc.M110.167023 PubMed 21169635
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