Khc73-GFP
(Plasmid
#31746)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMTWG
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKhc73
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SpeciesD. melanogaster (fly)
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Entrez GeneKhc-73 (a.k.a. Dmel_CG8183, CG8183, DmKIN73, DmKin73, DmKlp73, Dmel\CG8183, KHC-73, KIF 13A, KIF13A/B, KLP73, Khc 73, Khc73, Kin73, Kinesin-73, anon-EST:fe3C9, khc-73, khc73)
- Promoter Metallothionein
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MT1-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Khc73-GFP was a gift from Ron Vale (Addgene plasmid # 31746 ; http://n2t.net/addgene:31746 ; RRID:Addgene_31746) -
For your References section:
Kinesin-73 is a processive motor that localizes to Rab5-containing organelles. Huckaba TM, Gennerich A, Wilhelm JE, Chishti AH, Vale RD. J Biol Chem. 2011 Mar 4;286(9):7457-67. Epub 2010 Dec 18. 10.1074/jbc.M110.167023 PubMed 21169635