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pDONR223-MCU
(Plasmid #31726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2787
  • Modifications to backbone
    None
  • Vector type
    pDONR Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MCU
  • Alt name
    CCDC109A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1056
  • Mutation
    *
  • GenBank ID
    NM_138357
  • Entrez Gene
    MCU (a.k.a. C10orf42, CCDC109A, HsMCU)
  • Promoter None
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 (-21) Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Marc Vidal's lab originally made this construct (PMID15489335). Via collaboration we received it and sequence verified it for use in the paper associated with this entry. The Vidal/Mootha collaboration was cited in Pagliarini et al, 2008, Cell. (PMID 18614015)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments


*A3T aa residue substitution in MCU, which is not known to affect protein function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223-MCU was a gift from Vamsi Mootha (Addgene plasmid # 31726 ; http://n2t.net/addgene:31726 ; RRID:Addgene_31726)

  • For your References section:

    Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter. Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, Sancak Y, Bao XR, Strittmatter L, Goldberger O, Bogorad RL, Koteliansky V, Mootha VK. Nature. 2011 Jun 19. ():. 10.1038/nature10234 PubMed 21685886
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