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Addgene

pCB210
(Plasmid #31722)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 6018
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CUP9
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    CUP9 (a.k.a. YPL177C)
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • Cup9 promoter (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB210 was a gift from Alexander Varshavsky (Addgene plasmid # 31722 ; http://n2t.net/addgene:31722 ; RRID:Addgene_31722)

  • For your References section:

    The N-end rule pathway controls the import of peptides through degradation of a transcriptional repressor. Byrd C, Turner GC, Varshavsky A. EMBO J. 1998 Jan 2. 17(1):269-77. 10.1093/emboj/17.1.269 PubMed 9427760
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