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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4716
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTIMP3
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SpeciesH. sapiens (human)
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Entrez GeneTIMP3 (a.k.a. HSMRK222, K222, K222TA2, SFD)
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5M-TIMP3 was a gift from Rik Derynck (Addgene plasmid # 31715 ; http://n2t.net/addgene:31715 ; RRID:Addgene_31715) -
For your References section:
TACE-mediated ectodomain shedding of the type I TGF-beta receptor downregulates TGF-beta signaling. Liu C, Xu P, Lamouille S, Xu J, Derynck R. Mol Cell. 2009 Jul 10. 35(1):26-36. 10.1016/j.molcel.2009.06.018 PubMed 19595713