pRK5F-TACE
(Plasmid #31713)

• Purpose: Mammalian expression of human TACE (ADAM17)
• Depositing Lab: Rik Derynck
• Publication: Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4716
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TACE
• Species: H. sapiens (human)
• Entrez Gene: ADAM17 (a.k.a. ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA