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Addgene

SnailHA_pTK_CMV
(Plasmid #31700)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31700 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAdTrack-CMV
  • Backbone manufacturer
    B. Vogelstein
  • Backbone size w/o insert (bp) 9220
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Snail
  • Alt name
    SNAI1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    822
  • GenBank ID
    NM_005985
  • Entrez Gene
    SNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HIndIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains GFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SnailHA_pTK_CMV was a gift from Paul Wade (Addgene plasmid # 31700 ; http://n2t.net/addgene:31700 ; RRID:Addgene_31700)
  • For your References section:

    Aberrant expression of the transcription factors snail and slug alters the response to genotoxic stress. Kajita M, McClinic KN, Wade PA. Mol Cell Biol. 2004 Sep . 24(17):7559-66. 10.1128/MCB.24.17.7559-7566.2004 PubMed 15314165