SnailHA_pTK_CMV
(Plasmid
#31700)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31700 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAdTrack-CMV
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Backbone manufacturerB. Vogelstein
- Backbone size w/o insert (bp) 9220
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSnail
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Alt nameSNAI1
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SpeciesH. sapiens (human)
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Insert Size (bp)822
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GenBank IDNM_005985
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Entrez GeneSNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HIndIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains GFP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SnailHA_pTK_CMV was a gift from Paul Wade (Addgene plasmid # 31700 ; http://n2t.net/addgene:31700 ; RRID:Addgene_31700) -
For your References section:
Aberrant expression of the transcription factors snail and slug alters the response to genotoxic stress. Kajita M, McClinic KN, Wade PA. Mol Cell Biol. 2004 Sep . 24(17):7559-66. 10.1128/MCB.24.17.7559-7566.2004 PubMed 15314165
Map uploaded by the depositor.