SlugMyc_pcDNA3
(Plasmid #31698)

• Depositing Lab: Paul Wade
• Publication: Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Slug
• Alt name: SNAI2
• Species: H. sapiens (human)
• Insert Size (bp): 846
• GenBank ID: NM_003068
• Entrez Gene: SNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry