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Addgene

pcDNA3_FLAG_MTA3
(Plasmid #31696)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Metastasis associated protein 3
  • Alt name
    MTA3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1548
  • GenBank ID
    NM_020744
  • Entrez Gene
    MTA3
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3_FLAG_MTA3 was a gift from Paul Wade (Addgene plasmid # 31696 ; http://n2t.net/addgene:31696 ; RRID:Addgene_31696)

  • For your References section:

    MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA. Cell. 2003 Apr 18. 113(2):207-19. 10.1016/S0092-8674(03)00234-4 PubMed 12705869
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