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Addgene

Slug_pGL2
(Plasmid #31695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2_Basic
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slug
  • Alt name
    SNAI2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    906
  • GenBank ID
    NM_003068
  • Entrez Gene
    SNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains Slug promoter (-908 to -2) sequence upstream of luciferase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Slug_pGL2 was a gift from Paul Wade (Addgene plasmid # 31695 ; http://n2t.net/addgene:31695 ; RRID:Addgene_31695)

  • For your References section:

    MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA. Cell. 2003 Apr 18. 113(2):207-19. 10.1016/S0092-8674(03)00234-4 PubMed 12705869
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