pECE-M2-PAK2 S20A
(Plasmid #31681)

• Depositing Lab: Anne Brunet
• Publication: Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.

Backbone

• Vector backbone: pECE, Addgene plasmid 26453
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p21-activated protein kinase 2 S20A
• Alt name: M2-PAK2 S20A
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Mutation: changed Serine 20 to Alanine
• Entrez Gene: PAK2 (a.k.a. KNO2, PAK65, PAKgamma)
• Tag / Fusion Protein:
  • M2 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems Human Orfeome collection

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry