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pGEX-BAIAP2 S366A
(Plasmid #31676)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T3
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BAI1-associated protein 2 S366A
  • Alt name
    GST-BAIAP2 S366A
  • Alt name
    BAIAP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1550
  • Mutation
    changed Serine 366 to Alanine
  • Entrez Gene
    BAIAP2 (a.k.a. BAP2, FLAF3, IRSP53, WAML)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pGEX F
  • 3′ sequencing primer pGEX R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems Human Orfeome Collection

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-BAIAP2 S366A was a gift from Anne Brunet (Addgene plasmid # 31676 ; http://n2t.net/addgene:31676 ; RRID:Addgene_31676)

  • For your References section:

    Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network of Proteins Involved in Mitosis. Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat KM, Brunet A. Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1. 10.1016/j.molcel.2011.11.005 PubMed 22137581
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