pGEX-CDC27 S379A
(Plasmid #31674)

• Depositing Lab: Anne Brunet
• Publication: Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.

Backbone

• Vector backbone: pGEX-4T3
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: cell division cycle 27 homolog S379A
• Alt name: GST-CDC27 S379A
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: changed Serine 379 to Alanine
• Entrez Gene: CDC27 (a.k.a. ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: pGEX F
• 3′ sequencing primer: pGEX R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems Human Orfeome Collection

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry