pECE-HA-AMPKalpha2 wt
(Plasmid #31654)

• Depositing Lab: Anne Brunet
• Publication: Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.

Backbone

• Vector backbone: pECE, Addgene plasmid 26453
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: protein kinase, AMP-activated, alpha 2 catalytic subunit
• Alt name: PRKAA2
• Species: H. sapiens (human)
• Insert Size (bp): 1660
• Entrez Gene: PRKAA2 (a.k.a. AMPK, AMPK2, AMPKa2, PRKAA)
• Tag / Fusion Protein:
  • HA (YDVPDYASL) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry