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Addgene

pECE-M2-AMPKalpha2 M93G
(Plasmid #31653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECE, Addgene plasmid 26453
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G
  • Alt name
    PRKAA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1660
  • Mutation
    changed Methionine 93 to Glycine
  • Entrez Gene
    PRKAA2 (a.k.a. AMPK, AMPK2, AMPKa2, PRKAA)
  • Tag / Fusion Protein
    • M2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECE-M2-AMPKalpha2 M93G was a gift from Anne Brunet (Addgene plasmid # 31653 ; http://n2t.net/addgene:31653 ; RRID:Addgene_31653)

  • For your References section:

    Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network of Proteins Involved in Mitosis. Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat KM, Brunet A. Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1. 10.1016/j.molcel.2011.11.005 PubMed 22137581
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