pcDNA-N1-PDZ1-GAGA
(Plasmid #31651)

• Depositing Lab: Edward Weinman
• Publication: Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8.

Backbone

• Vector backbone: pcDNA3.1/Hygro (+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Alt name: NHERF
• Species: O. cuniculus (rabbit)
• Insert Size (bp): 1200
• Mutation: changed P(21)NGYGF changed to H(21)NGAGA
• GenBank ID: U19815
• Entrez Gene: SLC9A3R1
• Promoter: cmv
• Tag / Fusion Protein:
  • His, S-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: V= Nhe1 I=Xba1 (destroyed during cloning)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ERRATUM- Materials & Methods :This in NOT the mouse NHERF-1 cDNA.Paper should read "The rabbit cDNA was inserted into......".

The rabbit cDNA was cut from the pet30a vector & put into pcDNA. The his & S tag comes from the pet30a vector.
VECTOR UPDATED JAN14,2002
his tagged rabbit NHERF PDZ1 mutation PNGYGF to HNGAGA
pcDNA3.1/hygr(+) cut nhe1&xho1
pET 30ac_FMZ1.seq cut Xba1 & Xho1

his tag translation = 937