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Addgene

pcDNA-NHERF1
(Plasmid #31650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
    NHERF
  • Species
    O. cuniculus (rabbit)
  • Insert Size (bp)
    1200
  • Mutation
    none
  • GenBank ID
    u19815
  • Entrez Gene
    SLC9A3R1
  • Promoter CMV
  • Tag / Fusion Protein
    • His & S tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site V=Nhe1 I= Xba1 (destroyed during cloning)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer t7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ERRATUM- Materials & Methods :This in NOT the mouse NHERF-1 cDNA.Paper should read "The rabbit cDNA was inserted into......".

The rabbit cDNA was cut from the pet30a vector & put into pcDNA. The his & S tag comes from the pet30a vector.

Translation NHERF-1= bp #937

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-NHERF1 was a gift from Edward Weinman (Addgene plasmid # 31650 ; http://n2t.net/addgene:31650 ; RRID:Addgene_31650)

  • For your References section:

    A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange. Weinman EJ, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S. Biochemistry. 2003 Nov 4. 42(43):12662-8. 10.1021/bi035244l PubMed 14580213
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