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Addgene

Rabbit NHERF-1
(Plasmid #31644)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
    NHERF
  • Species
    O. cuniculus (rabbit)
  • Insert Size (bp)
    1895
  • Mutation
    none
  • GenBank ID
    u19815
  • Entrez Gene
    SLC9A3R1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer M13 T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rabbit NHERF-1 was a gift from Edward Weinman (Addgene plasmid # 31644 ; http://n2t.net/addgene:31644 ; RRID:Addgene_31644)

  • For your References section:

    Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger. Weinman EJ, Steplock D, Wang Y, Shenolikar S. J Clin Invest. 1995 May . 95(5):2143-9. 10.1172/JCI117903 PubMed 7738182
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