MSCV-SNAP-Smo
(Plasmid
#31619)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV-pac
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Backbone manufacturerHawley et al., 1994
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression, Retroviral
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Selectable markerspuromycin N-acetyl transferase (pac)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmo
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SpeciesM. musculus (mouse)
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Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
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Tag
/ Fusion Protein
- SNAP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-SNAP-Smo was a gift from Rajat Rohatgi (Addgene plasmid # 31619 ; http://n2t.net/addgene:31619 ; RRID:Addgene_31619) -
For your References section:
Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium. Milenkovic L, Scott MP, Rohatgi R. J Cell Biol. 2009 Nov 2. 187(3):365-74. 10.1083/jcb.200907126 PubMed 19948480