-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCS2+
-
Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4095
-
Vector typeMammalian Expression, Worm Expression ; Zebrafish, Avian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameSmo
-
SpeciesM. musculus (mouse)
-
Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
-
Tag
/ Fusion Protein
- SNAP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Wild-type mouse Smo was tagged at the N terminus (with the insertion C terminal to the signal sequence) with SNAP.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS2-SNAP-Smo was a gift from Rajat Rohatgi (Addgene plasmid # 31617 ; http://n2t.net/addgene:31617 ; RRID:Addgene_31617) -
For your References section:
Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium. Milenkovic L, Scott MP, Rohatgi R. J Cell Biol. 2009 Nov 2. 187(3):365-74. 10.1083/jcb.200907126 PubMed 19948480