pcDNA3.1-HisFlag-CAD
(Plasmid #31615)

• Depositing Lab: Aziz Sancar
• Publication: Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-30.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAD
• Alt name: carbamoyl phosphate synthetase/aspartate transcarbamoylase/dihydroorotase
• Species: hamster
• Insert Size (bp): 6600
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • T7 (N terminal on insert)
  • 3xFlag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lee Graves
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry