pcDNA3-ATR Kinase dead
(Plasmid #31612)

• Depositing Lab: Aziz Sancar
• Publication: Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATR
• Alt name: ATM and Rad3 Related
• Species: H. sapiens (human)
• Insert Size (bp): 8000
• Mutation: K2327R (kinase dead)
• Entrez Gene: ATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Robert Abraham
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains an R1631H polymorphism. This is not thought to affect plasmid function.