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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31610 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHMGB2
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SpeciesM. musculus (mouse)
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Insert Size (bp)640
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Entrez GeneHmgb2 (a.k.a. Hmg2, HMG-2, C80539, MGC103184)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 Flag mHMGB2 was a gift from Yasuhiko Kawakami (Addgene plasmid # 31610 ; http://n2t.net/addgene:31610 ; RRID:Addgene_31610) -
For your References section:
Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Taniguchi N, Carames B, Kawakami Y, Amendt BA, Komiya S, Lotz M. Proc Natl Acad Sci U S A. 2009 Sep 29. 106(39):16817-22. 10.1073/pnas.0904414106 PubMed 19805379