Skip to main content
Addgene

pcDNA3.1 Flag hHMGB1
(Plasmid #31609)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31609 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HMGB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    650
  • Entrez Gene
    HMGB1 (a.k.a. HMG-1, HMG1, HMG3, SBP-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 Flag hHMGB1 was a gift from Yasuhiko Kawakami (Addgene plasmid # 31609 ; http://n2t.net/addgene:31609 ; RRID:Addgene_31609)
  • For your References section:

    Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Taniguchi N, Carames B, Kawakami Y, Amendt BA, Komiya S, Lotz M. Proc Natl Acad Sci U S A. 2009 Sep 29. 106(39):16817-22. 10.1073/pnas.0904414106 PubMed 19805379