pKin1A
(Plasmid #31607)

• Depositing Lab: Anthony Brown
• Publication: Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-5006.

Backbone

• Vector backbone: pEGFP-C1 (EGFP removed)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3940
• Modifications to backbone: Deleted EGFP (601-1390) from original pEGFP-C1 vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli DH5α, 16hrs at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kinesin-1A
• Alt name: KIF5A
• Species: M. musculus (mouse)
• Insert Size (bp): 3564
• GenBank ID: BC058396
• Entrez Gene: Kif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: specific sequence primer
• 3′ sequencing primer: specific sequence primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kinesin-1A cDNA (GenBank accession BC058396, I.M.A.G.E. clone:6824963, MGC:67074) was obtained from American Type Culture Collection (Manassas, VA)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry