pKin1B
(Plasmid #31604)

• Depositing Lab: Anthony Brown
• Publication: Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-5006.

Backbone

• Vector backbone: pEGFP-C1 (EGFP removed)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3941
• Modifications to backbone: Deleted EGFP (601-1390) from original pEGFP-C1 vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli DH5α, 16hrs at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: kinesin-1B
• Alt name: KIF5B
• Species: M. musculus (mouse)
• Insert Size (bp): 3332
• GenBank ID: BC090841
• Entrez Gene: Kif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: specific sequence primer
• 3′ sequencing primer: specific sequence primer

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Kinesin-1B cDNA (GenBank accession BC090841, I.M.A.G.E. clone:30543821, MGC:102147) was obtained from Open Biosystems (Huntsville, AL)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Because the insert may be toxic to the bacterial strain, we recommend pooling and concentrating DNA preps.