pHA-ΔN331-Kin1B
(Plasmid #31603)

• Depositing Lab: Anthony Brown
• Publication: Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-5006.

Backbone

• Vector backbone: pCGN
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli DH5α, 16hrs at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ΔN331 kinesin-1B
• Alt name: headless kinesin-1B
• Alt name: headless KIF5B
• Species: M. musculus (mouse)
• Insert Size (bp): 2168
• Mutation: deleted amino acids 1-331
• GenBank ID: BC090841
• Entrez Gene: Kif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: specific sequence primer
• 3′ sequencing primer: specific sequence primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I.M.A.G.E. clone 30543821 obtained from Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry