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Addgene

pOVA
(Plasmid #31598)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31598 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    TCAE (modified)
  • Backbone manufacturer
    Reff et al., 1994
  • Backbone size w/o insert (bp) 9209
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OVA
  • Alt name
    ovalbumin
  • Species
    G. gallus (chicken)
  • Entrez Gene
    OVAL (a.k.a. OVA, SERPINB14)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    OVA was a gift of M. Moore, Genentech, South San Francisco, CA, and M. Bevan, University of Seattle, Seattle, WA
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOVA was a gift from Shoshana Levy (Addgene plasmid # 31598 ; http://n2t.net/addgene:31598 ; RRID:Addgene_31598)
  • For your References section:

    DNA vaccination with cytokine fusion constructs biases the immune response to ovalbumin. Maecker HT, Umetsu DT, DeKruyff RH, Levy S. Vaccine. 1997 Oct . 15(15):1687-96. 10.1016/S0264-410X(97)00088-1 PubMed 9364701