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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTCAE (modified)
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Backbone manufacturerReff et al., 1994
- Backbone size w/o insert (bp) 9209
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOVA
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Alt nameovalbumin
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SpeciesG. gallus (chicken)
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Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOVA was a gift of M. Moore, Genentech, South San Francisco, CA, and M. Bevan, University of Seattle, Seattle, WA
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
*The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOVA was a gift from Shoshana Levy (Addgene plasmid # 31598 ; http://n2t.net/addgene:31598 ; RRID:Addgene_31598) -
For your References section:
DNA vaccination with cytokine fusion constructs biases the immune response to ovalbumin. Maecker HT, Umetsu DT, DeKruyff RH, Levy S. Vaccine. 1997 Oct . 15(15):1687-96. 10.1016/S0264-410X(97)00088-1 PubMed 9364701