pQStrep2-PREI3
(Plasmid #31592)

• Depositing Lab: Konrad Buessow
• Publication: Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Backbone

• Vector backbone: pQStrep2
• Backbone size w/o insert (bp): 3460
• Modifications to backbone: GenBank: AY028642
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: preimplantation protein 3
• Alt name: PSFEp758B0812
• Alt name: PREI3
• Alt name: CGI-95
• Species: H. sapiens (human)
• Insert Size (bp): 744
• GenBank ID: DQ000500
• Entrez Gene: MOB4 (a.k.a. 2C4D, CGI-95, MOB1, MOB3, MOBKL3, PHOCN, PREI3)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • StrepII-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: TGAGCGGATAACAATTTCACACAG
• 3′ sequencing primer: GGCAACCGAGCGTTCTGAAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is a small gap between Addgene's quality control sequence and the reference sequence provided by the depositor. The gap is downstream of the ORF and should not affect function.