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Addgene

pSYC-97
(Plasmid #31565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS4
  • Backbone size w/o insert (bp) 4146

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCS4+-NLS-EGFP-P2A-mCherry-CAAX
  • Alt name
    pCS4+-NLS-EGFP-P2A-mCherry-CAAX
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1652

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site AflII (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSYC-97 was a gift from Seok-Yong Choi (Addgene plasmid # 31565 ; http://n2t.net/addgene:31565 ; RRID:Addgene_31565)

  • For your References section:

    High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice. Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, Choi SY. PLoS One. 2011 . 6(4):e18556. 10.1371/journal.pone.0018556 PubMed 21602908
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