pT-TK
(Plasmid #31549)

• Depositing Lab: Yegor Vassetzky
• Publication: Dmitriev et al BMC Res Notes. 2008 . 1():135.

Backbone

• Vector backbone: phRL-TK
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4113
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LoxP
• Alt name: LoxP site
• Insert Size (bp): 20

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspHI (destroyed during cloning)
• 3′ cloning site: BspHI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: LoxP site was taken from phrGFP plasmid (Stratagene)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To replace hRLuc gene use NheI/XbaI digestion.
Nucleotide mismatches between Addgene QC and depositor full plasmid sequence do not effect plasmid function.