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Addgene

pcDNA3-YFP-PKD1
(Plasmid #31527)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-HA-Protein Kinase D
  • Alt name
    YFP-PKD
  • Alt name
    PKD
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRKD1 (a.k.a. CHDED, PKC-MU, PKCM, PKD, PRKCM)
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It should work fine with the 12CA5 HA antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-YFP-PKD1 was a gift from Alexandra Newton (Addgene plasmid # 31527 ; http://n2t.net/addgene:31527 ; RRID:Addgene_31527)

  • For your References section:

    The protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. Kunkel MT, Garcia EL, Kajimoto T, Hall RA, Newton AC. J Biol Chem. 2009 Sep 4. 284(36):24653-61. 10.1074/jbc.M109.024547 PubMed 19581308
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