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MDH1-miR-221-PGK-GFP-Blast
(Plasmid #31519)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miR-221
  • Alt name
    miR221
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mir221 (a.k.a. Mirn, Mirn221, mir-221, mmu-mir-2, mmu-mir-221)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MDH1-miR-221-PGK-GFP-Blast was a gift from Chang-Zheng Chen (Addgene plasmid # 31519 ; http://n2t.net/addgene:31519 ; RRID:Addgene_31519)

  • For your References section:

    MicroRNA programs in normal and aberrant stem and progenitor cells. Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R, Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen CZ. Genome Res. 2011 May . 21(5):798-810. 10.1101/gr.111385.110 PubMed 21451113
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