MDH1-miR-196a-1SM-PGK-GFP-Blast
(Plasmid
#31510)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemiR-196a1
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Alt namemiR196a1
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SpeciesM. musculus (mouse)
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Mutationseed mutant control
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Entrez GeneMir196a-1 (a.k.a. Mirn196, Mirn196a-1, miR-196, mir-196a-1)
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer H1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MDH1-miR-196a-1SM-PGK-GFP-Blast was a gift from Chang-Zheng Chen (Addgene plasmid # 31510 ; http://n2t.net/addgene:31510 ; RRID:Addgene_31510) -
For your References section:
MicroRNA programs in normal and aberrant stem and progenitor cells. Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R, Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen CZ. Genome Res. 2011 May . 21(5):798-810. 10.1101/gr.111385.110 PubMed 21451113