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Addgene

L4440-daf-16af
(Plasmid #31506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L4440
  • Backbone size w/o insert (bp) 2790
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    daf-16a/f common
  • gRNA/shRNA sequence
    see View Sequences link below
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    328
  • Entrez Gene
    daf-16 (a.k.a. CELE_R13H8.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4440-daf-16af was a gift from Heidi Tissenbaum (Addgene plasmid # 31506 ; http://n2t.net/addgene:31506 ; RRID:Addgene_31506)

  • For your References section:

    A new DAF-16 isoform regulates longevity. Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA. Nature. 2010 Jul 22. 466(7305):498-502. 10.1038/nature09184 PubMed 20613724
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