L4440-daf-16b
(Plasmid #31504)

• Depositing Lab: Heidi Tissenbaum
• Publication: Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Backbone

• Vector backbone: L4440
• Backbone size w/o insert (bp): 2790
• Vector type: RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: daf-16b specific
• gRNA/shRNA sequence: see View Sequences link below
• Species: C. elegans (nematode)
• Insert Size (bp): 531
• Entrez Gene: daf-16 (a.k.a. CELE_R13H8.1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: M13-F20

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results found two nucleotide mismatches at bp# 450 & 525 when compared to the sequence provided by the depositing scientist. These mismatches are not a concern for the function of the plasmid.