pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
(Plasmid #31483)

• Depositing Lab: Andrew Scharenberg
• Publication: Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.

Backbone

• Vector backbone: pCVL
• Backbone size w/o insert (bp): 5000
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
• Insert Size (bp): 2939
• Mutation: Traffic Light 2.0 series have a +3 PEST sequence that aids in degradation of the +3 "gibberishFP" produced by mutNHEJ events. The mCherry sequence has M9S and M16L mutations that reduce background fluorescence from internal start codons. This is what ".1" stands for.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry